Equally impressive are the presentations of newer approaches, which take advantage of advances on the experimental front e. A theoretical study on the measurability of Bijvoet differences.
The solvent contrast variation method. Ab initio determination of a low resolution molecular envelope.
Direct methods in relation to isomorphous data. Phase refinement.
Density modification and direct methods. SHELX applications to macromolecules.
In the approaches discussed above it was supposed that the atom can occupy every point in the tested region with the same probability. This article is cited by 76 publications. Examples of applications of SAS to different types of biomols. Maltsev, Alexander Grishaev, and Ad Bax. ProtSkin , converts a protein sequence alignment to a property file used to map the sequence conservation onto the structure.
Model based very low resolution phasing. Use of noncrystallographic symmetry for ab initio phasing of virus.
Developments in maximum entropy and likelihood. Reinvestigation of the use of Patterson maps to extrapolate data to higher.
This book of the proceedings of the NATO Advanced Study Institute ASI on Direct Methods for Solving Macromolecular Structures was assembled from the lecturers' contributions and represents a comprehensive and in-depth overview of crystallographic structure determination methods for macromolecules.
While having a focus based on the direct methods, the Institute adopted an inclusive and broad perspective.
This book of the proceedings of the NATO Advanced Study Institute (ASI) on Direct Methods for Solving Macromolecular Structures was assembled from. Direct Methods for Solving Macromolecular Structures by Suzanne Fortier, , available at Book Depository with free delivery worldwide.
Thus, both direct and experimental phasing techniques are presented in this book, highlighting their complementarities and synergies. As weil, methodologies spanning the full crystallographic image reconstruction process - from low resolution envelope definition to high resolution atomic refinement- are discussed.
The first part of the book introduces the array of tools currently used in structure determination, whether originating from a mathematical, computational or experimental framework.
This section of the book displays the variety and ingenuity of old and new phasing approaches developed to solve increasingly complex structures. A case in point is the re-implementation of Buerger's superposition approach, which is now solving protein structures. Another beautiful example is found in the introduction to the traditional multiple isomorphous replacement approach where new techniques, such as site-directed mutagenesis and the use of inert gases in the preparation of heavy atom derivatives, are described.